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KMID : 0380620210530010034
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Korean Journal of Food Science and Technology
2021 Volume.53 No. 1 p.34 ~ p.39
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Anti-cancer effects of Salicornia herbacea extract in OVCAR-3 cells by induction of apoptosis pathway
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La Yu-Ri
Lee You-Rim
Lee Dong-Seok
Kim Soo-Hwan
Lee Hyeong-Seon
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Abstract
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This study investigated the anti-cancer effects of Salicornia herbacea L. fractions in human ovarian cancer cells (OVCAR-3). S. herbacea powder was extracted with 95% EtOH and sequentially fractionated with hexane, dichloromethane (DCM), ethyl acetate, butanol, and H2O. Further, the growth inhibitory effects of the six fractions were determined using the MTS assay. The DCM fraction dramatically decreased cell viability. Similarly, the cell cycle was arrested at the sub- G1 phase in DCM-treated cells. To confirm apoptosis, the cells were stained with annexin V/FITC-PI solution. Total, early, and late apoptotic cells were significantly increased in the DCM fraction. The mRNA expression of Bcl-2 was reduced, whereas the pro-apoptotic factors Bax and Bak were increased in DCM fraction-treated cells. These results indicated that the DCM fraction in S. herbacea exhibited strong apoptotic effects through the p53-dependent signaling pathway.
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KEYWORD
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Salicornia herbacea L., apoptosis, OVCAR-3 cells, DCM fraction
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